Part:BBa_K3245010:Design

A gene cluster that produces microcin B17, a kind of antimicrobial peptide.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2611
  Illegal BamHI site found at 1
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2366
  Illegal AgeI site found at 2538
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5471
  

Design Notes

We substituted the -35 to -10 region of the original promoter luxpR-HS with the one of J23100 / J23106, a strong constitutive promoter.

Source

Derivated from the luxpR-HS promoter designed by 2018 Tsinghua team.